Figure 9.

Confocal microscopic images of H₂O₂ formation in roots using H2DCFDA staining (A–F) and cell viability test (G–L) by propidium iodide staining, was performed on 30 days old roots of Indian mustard (Brassica juncea L.). Plants were grown with/without Cu stress and treated during pre-germination for 3 h with water, SNP, FCy (SNP analogue) and SNP + cPTIO (cPTIO as a specific NO-scavenger). Control: A and G; B and H: Cu; C and I: SNP; D and J: SNP+Cu; E and K: FCy + Cu; F and L: SNP+cPTIO+Cu.