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. 2020 Jun 4;10(6):856. doi: 10.3390/biom10060856

Figure 3.

Figure 3

PfAdoMetDC co-produced with PfHsp70-1 and KPf is structurally unique secondary structure from that co-produced with DnaK. Amino acid compositions of AdoMetDC of H. sapiens, E. coli and P. falciparum origins (a). PfAdoMetDC was co-produced either alone (NC) or in the presence of supplementary chaperone sets: DnaK + DnaJ (J + K); DnaK + DnaJ + GroEL (J + K + EL); PfHsp70-1+DnaJ (J + Pf); PfHsp70 + DnaJ + GroEL (J + Pf + EL); KPf + DnaJ (J + KPf); and KPf + DnaJ + GroEL (J + KPf + EL), respectively. The protein was then subjected to SEC-FPLC analysis (b); and far-UV CD spectrometric assays (c–f). CD spectrometric analysis was conducted either in the absence of substrate or in the presence of SAM (‘S’), the PfAdoMetDC substrate. The CD spectrum of PfAdoMetDC was presented as molar residue ellipticity (MRE; θ).