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. 2020 Jun 9;10(6):884. doi: 10.3390/biom10060884

Figure 2.

Figure 2

Mutp53-driven secretome mediates anti-apoptotic, anti-autophagic and chemoresistance effects. (A) Apoptosis was determined by the annexinV/FITC binding assay in AsPC-1 cultivated with mutp53-derived CM. The annexinV-FITC fluorescence intensity was measured by using a multimode plate reader and reported as arbitrary units (a.u.). (B) Autophagosome formation assay was determined by intracellular staining using the MDC probe in AsPC-1 cultivated with mutp53-derived CM. Statistical analysis * p < 0.05 CM-R175H vs CM-Mock; # p < 0.05 CM-R273H vs CM-Mock. (C) Cell growth was analyzed by Cristal Violet assay in AsPC-1 cultivated with mutp53-derived CM treated with 1 μM GEM for 48 h. Statistical analysis ^ p < 0.05 CM-R273H + GEM vs CM-Mock + GEM; ° p < 0.05 CM-Mock + GEM vs CM-Mock.