Table 1.
Comparison of the POCKIT DENV Reagent Set with a laboratory-developed rRT-PCR
| DENV rRT-PCR | Viral load | Results, POCKIT DENV | ||
|---|---|---|---|---|
| Result | N | Median (range)* | Detected, n | Sensitivity (%) |
| DENV positive | 78 | 5.2 (2.7–10.8) | 68 | 87.2 |
| DENV-1 | 24 | 5.3 (2.7–9.3) | 20 | 83.3 |
| DENV-2 | 33 | 4.4 (2.8–10.8) | 29 | 87.9 |
| DENV-3 | 18 | 6.8 (4.2–9.5) | 16† | 88.9 |
| DENV-4 | 3 | 4.8 (4.8–5.5) | 3 | 100 |
| DENV negative | 55 | – | 1‡ | – |
DENV = dengue virus; rRT-PCR = real-time reverse transcriptase PCR.
Determined by rRT-PCR, expressed as log10 copies/mL of serum.
One eluate each from plasma and whole blood tested negative (viral loads 4.2 and 5.4 log10 copies/mL, respectively).
Specificity, 98.2%.