Table 2.
Evaluation of POCKIT YFV reagent set
| YFV real-time reverse transcriptase PCR | POCKIT yellow fever | ||
|---|---|---|---|
| Sample | Viral load, range* | Detected, n/total (%) | Detected, n/total (%) |
| Sensitivity evaluation | ≥ 5.5 | 22/22 (100) | 22/22 (100) |
| 3.25–5.49 | 14/14 (100) | 14/14 (100) | |
| YFV strains† | 2.5–3.24 | 8/12 (66.7) | 7/12 (58.3) |
| 1.0–2.49 | 5/12 (41.7) | 1/12 (8.3) | |
| Specificity evaluation | |||
| Flaviviral strains‡ | 7.2–8.8 | 0/8 | 0/8 |
| DENV-positive samples§ | 5.1–9.3 | 0/12 | 0/12 |
| DENV-negative samples | – | 0/12 | 0/12 |
DENV = dengue virus; YFV = yellow fever virus.
Expressed as log10 copies/mL of sample, estimated for YFV strains and calculated for DENV serum samples.
Yellow fever virus strains: Asibi–Ghana, 1927 and Senegal ArD 149194, 1996 (West Africa II genotype); Couma–Ethiopia 1961 (East/Central Africa); Angola SH 281788, 2016 (Angola); INHRR 10a-10 – Venezuela 2010 (South America I); and FVB 0196 – Bolivia 2006 (South America II).
Genomic RNA from West Nile virus (two strains), tick-borne encephalitis virus, Japanese encephalitis virus, St. Louis encephalitis virus (two strains), Zika virus, and DENV-4.
DENV-1 (n = 11) and DENV-4 (n = 1); a single DENV-1 sample yielded an initial indeterminate POCKIT YFV result that was negative on repeat testing (DENV viral load, 8.1 log10 copies/mL of serum).