Figure 1.

Overview of study design illustrating the infection of mosquitoes, followed by the independent assessments of in vitro transmission in mosquitoes and NHP infection over a 28-day time course. The schematic diagram illustrates the infection and sampling of mosquitoes and NHPs, sampling time points, and steps involved in each part of the study. Viral infection and incubation of mosquitoes took place from day 14–day 0 (in green, left hand side). The top branch of the diagram (in blue) shows the major steps involved in the IVT assay. The IVT assay was performed in parallel with the NHP infections, shown on the bottom branch of the figure (in red). Voluntary blood samples were collected from NHPs to test for evidence of dengue virus infection. Each experiment involved three NHPs, each fed upon by three mosquitoes, and the whole experiment was replicated three times (meaning a total of nine NHPs and 27 mosquitoes). IVT = in vitro transmission; NHP = nonhuman primate.