Figure 2.
Infection results for mosquitoes tested in the in vitro transmission (IVT) assay, indicating the viral load in the salivary glands and the transmission status of each mosquito. Each data point represents the log10 viral load of the salivary glands from a single mosquito, as measured by RT-PCR. These points are plotted against the nonhuman primate (NHP) on which they fed (3 mosquitoes for each NHP). Mosquitoes were tested for IVT 1 day after they had fed upon the NHPs. Each data point represents a single mosquito that was exposed to virus and fed upon one of the NHPs. Closed colored circles (●) represent mosquitoes with evidence of virus in their saliva in the IVT assay, open colored diamonds (◇) represent mosquitoes with no evidence of virus transmission, crosses (✕) represent mosquitoes for which no data are available about their transmission status (i.e., all the mosquitoes that were inoculated with their saliva died before sampling). The smaller black dot (•) represents the average viral titer in the salivary glands of those three mosquitoes that fed upon each respective NHP.