Table 2.
List of the differentially expressed proteins identified in human plasma between hyperthyroid and euthyroid states, after treatment with antithyroid medication, using two-dimensional difference in gel electrophoresis (2D-DIGE). The differences in fold change are shown. Protein name, accession number, Mascot score, mass spectrometry (MS)% coverage, protein molecular weight (MW), and isoelectric point (pI) values according to the UniProt database are listed. ANOVA, analysis of variance.
| Spot No | Accession No a | Protein Name | MASCOT ID | Pi b | MW c | Cov% | Score d | p-Value (ANOVA) | Ratio Hyper/Control | EXP e |
|---|---|---|---|---|---|---|---|---|---|---|
| 1153 | P00738 | Haptoglobin | HPT_HUMAN | 6.13 | 45861 | 23 | 78 | 0.01 | 1.5 | UP |
| 729 | P01876 | Ig alpha-1 chain C region | IGHA1_HUMAN | 6.08 | 38486 | 42 | 58 | 0.01 | 1.5 | UP |
| 1149 | P00738 | Haptoglobin | HPT _HUMAN | 6.13 | 45861 | 27 | 69 | 0.01 | 1.7 | UP |
| 425 | P13671 | Complement component C6 | CO6_HUMAN | 6.39 | 108367 | 25 | 57 | 0.05 | 1.5 | UP |
| 966 | O14791 | Apolipoprotein L1 | APOL1_HUMAN | 5.60 | 44004 | 32 | 59 | 0.05 | 1.5 | UP |
| 855 | P02750 | Leucine-rich alpha-2-glycoprotein | A2GL_HUMAN | 6.45 | 38382 | 42 | 71 | 0.05 | 1.5 | UP |
| 1097 | P02647 | Apolipoprotein A-I | APOA1_HUMAN | 5.56 | 30759 | 72 | 238 | 0.05 | 1.5 | DOWN |
| 674 | P02679 | Fibrinogen gamma chain | FIBG_HUMAN | 5.37 | 52106 | 50 | 102 | 0.012 | 1.5 | UP |
| 348 | P00747 | Plasminogen | PLMN_HUMAN | 7.04 | 93247 | 30 | 77 | 0.016 | 1.8 | DOWN |
| 776 | P01009 | Alpha-1-antitrypsin | A1AT_HUMAN | 5.37 | 46878 | 40 | 78 | 0.039 | 1.7 | DOWN |
| 965 | P01009 | Alpha-1-antitrypsin | A1AT_HUMAN | 5.37 | 46878 | 36 | 68 | 0.047 | 1.5 | DOWN |
| 958 | P10909 | Clusterin | CLUS_HUMAN | 5.89 | 53031 | 26 | 78 | 0.053 | 1.5 | UP |
| 763 | P02675 | Fibrinogen beta chain | FIBB_HUMAN | 8.54 | 56577 | 55 | 205 | 0.050 | 1.5 | DOWN |
| 435 | P00736 | Complement C1r subcomponent | C1R_HUMAN | 5.82 | 81606 | 26 | 99 | 0.051 | 1.7 | DOWN |
| 780 | P00736 | Complement C1r subcomponent | C1R_HUMAN | 5.82 | 81606 | 33 | 115 | 0.0268 | 1.8 | DOWN |
| 560 | Q14624 | Inter-alpha-trypsin inhibitor heavy chain H4 | ITIH4_HUMAN | 6.51 | 103521 | 37 | 85 | 0.0386 | 1.5 | DOWN |
| 1339 | P04217 | Alpha-1B-glycoprotein | A1BG_HUMAN | 5.58 | 54809 | 37 | 90 | 0.016 | 1.5 | UP |
| 1591 | P02679 | Fibrinogen gamma chain | FIBG_HUMAN | 5.37 | 52106 | 50 | 129 | 0.0372 | 1.5 | UP |
| 849 | P15169 | Carboxypeptidase N catalytic chain | CBPN_HUMAN | 6.86 | 52538 | 33 | 62 | 0.021 | 1.5 | UP |
| 835 | P02790 | Hemopexin | HEMO_HUMAN | 6.55 | 52385 | 48 | 123 | 0.025 | 1.5 | UP |
a Protein accession number for SWISSPROT Database. b Theoretical isoelectric point. c Theoretical relative mass. d MASCOT score. e Protein expression between hypothyroid and euthyroid states.