Figure 3.

IgG antibodies subclass titer analysis. The IgG antibodies subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3 induced in BALB/c (A) and C57BL/6 (B) mice were measured by ELISA against PvCSP-All_FL and PvAMA-1. The results are expressed as the arithmetic mean titers of each group in log₁₀ ± SEM and were statistically compared using one-way ANOVA followed by Tukey’s test for multiple comparisons. Significant differences between groups are denoted on the graph: * p < 0.05, ** p < 0.01, and *** p < 0.001. Nonsignificant (ns) differences are indicated (p > 0.05). The titers were used to calculate the IgG1/IgG2a (A) and IgG1/IgG2c (B) ratio, and the ratios are indicated above the plot according to the groups.