Figure 5.

Lymphocyte proliferation from C57BL/6 mice immunized with formulation containing PvCSP-All_FL, PvAMA-1, and poly(I:C) adjuvant. Pooled splenocytes were collected from immunized C57BL/6 mice. The cells stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) were plated and stimulated (for five days) with homologous proteins or one of the PvCSP variants (10 μg) or pooled synthetic peptides (26 μg), which covered the entire length of the C- or N-terminal PvCSP or mitogen-concanavalin A (2.5 μg). The events were acquired in the FACSCanto II and analyzed using the software FlowJo^®. The results are expressed as percentage (%) of TCD4⁺ (A) or TCD8⁺ (B) proliferative cells. These data are representative of two independent assays from a pool of three immunized mice.