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. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Sci Transl Med. 2020 Mar 25;12(536):eaaw3210. doi: 10.1126/scitranslmed.aaw3210

Figure 3. The translaminar pressure difference drives ocular glymphatic outflow.

Figure 3.

(A) Schematic of the setup used for analyzing hAβ transport following intravitreal injection while manipulating ICP. Top: Mean ICP and IOP normalized to control (± SEM) plotted as a function of time in the high, normal, and low ICP groups (n = 10-12). (B) Representative background-subtracted heat-maps of hAβ in the optic nerve from high, normal, and low ICP groups. (C) Top: Averaged fluorescent intensity profiles of hAβ in the optic nerves from the three groups. Bottom: The distance of tracer transport (n = 6-8, ***P < 0.001, n.s. P = 0.9976 one-way ANOVA followed by Tukey’s post hoc test). (D-E) Total hAβ signal and peak intensities in the optic nerve 30 min after intravitreal injection (n = 6-8 for each group, *P < 0.05, **P < 0.01, ****P < 0.0001, n.s. P = 0.2996 one-way ANOVA followed by Tukey’s post hoc test). (Scale: B: 500μm).