Table 1.
Summary of the de novo assembly of transcriptome sequence reads from the TD- and MH-grown plants. TD and MH indicated high-altitude and low-altitude samples collected from the Tongde and Minhe regions located in the Qinghai-Tibetan Plateau, respectively. Q20 and Q30 are referred to the base call accuracy rate of 99% and 99.9%, respectively. N50 is defined as the read length when such reads added to all the longer reads reach to 50% sum of reads of all lengths.
| Content | Total | TD-1 | TD-2 | TD-3 | MH-1 | MH-2 | MH-3 |
|---|---|---|---|---|---|---|---|
| Total raw reads | 57,014,250 | 44,392,764 | 81,922,056 | 69,023,986 | 53,001,304 | 44,778,542 | 48,966,846 |
| Total clean reads | 55,645,070 | 43,177,362 | 79,839,438 | 67,258,662 | 51,867,538 | 43,809,044 | 47,918,378 |
| GC percentage (%) | 43.25% | 43.43% | 44.07% | 44.08% | 43.67% | 43.94% | |
| Clean reads Q20 (%) | 97.51% | 97.66% | 97.66% | 97.76% | 97.76% | 97.80% | |
| Clean reads Q30 (%) | 93.59% | 93.95% | 93.95% | 94.09% | 94.14% | 94.22% | |
| Clean reads (%) | 97.26% | 97.46% | 97.44% | 97.86% | 97.83% | 97.86% | |
| Total mapped reads (%) | 88.04% | 88.71% | 89.16% | 89.54% | 88.91% | 89.33% | |
| Total expressed genes | 74,791 | 64,331 | 62,887 | 59,219 | 56,903 | 60,218 | 60,218 |
| Total transcripts | 218,482 | ||||||
| Total unigenes | 74,791 | ||||||
| N50 (bp) | 1458 | ||||||
| Mean length of unigenes (bp) | 820 |