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. 2020 Jun 17;9(6):757. doi: 10.3390/plants9060757

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of Abeliophyllum distichum leaves extract (AL)-induced apoptosis and ROS production in SK-MEL-2 cells. (A) Early apoptotic cells and dead cells (late-stage apoptotic or necrotic cells) were analyzed using a fluorescence reader after Annexin V and PI staining. (B) Intracellular ROS generation was detected in SK-MEL-2 cells after treatment with different concentrations of AL by staining with DCFH-DA. The data are expressed as means ± SE. Values with different superscript letters are significantly different (p < 0.05).