Skip to main content
. 2020 Jul 13;16(7):e9427. doi: 10.15252/msb.20199427

Figure 5. Evolved CasTA functions in multiple bacterial species.

Figure 5

  1. Multiple sequence alignment of AsiA homologs from different phage genomes at residue positions 50–61. Highlighted red residues indicate positions that are mutated in AsiA‐m2.1.
  2. CRISPRi in S. enterica and K. oxytoca using CasTA2.1.
  3. CRISPRa in S. enterica and K. oxytoca using CasTA1.0 (dCas9‐AsiA_wt), CasTA2.1 (dCas9‐AsiA_m2.1) or ancestral strain with basal promoter expression (none).
Data information: All data are 3–4 biological replicates with ± SEM. *Student's t‐test, P < 0.0001 NS = non‐significant.