Skip to main content
. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Invest New Drugs. 2011 Sep 20;30(5):1849–1864. doi: 10.1007/s10637-011-9746-x

Fig. 8.

Fig. 8

Inhibition of MMP2 mRNA expression and protein secretion by synthetic APF derivatives. T24, 5637, ACHN, A549, PANC-1, HCC1428, and A375 cells were serum-starved, then treated with 500 nM as-APF (“APF”) or negative control peptide (“PEP”) for 72 h prior to collection of supernatant and mRNA extraction. a Western blot of supernatant proteins; GAPDH was used as a control for secreted cell proteins. b qRT-PCR of cell mRNA. qRT-PCR was performed in duplicate on three separate occasions for each sample; data are expressed as the mean change in normalized mRNA copy number following treatment with as-APF (+/− standard deviation) as compared to negative control peptide (the latter set to “1”). (p=.001 for T24 cells; p=.002 for A549 cells; p=.003 for PANC-1 cells; p=.03 for 5637 cells; p=.04 for A375 cells)