Table 1.

Effect of treatment start day on the efficacy of vorinostat

Treatment start	n	Micrometastases		Large metastases*
		Mean† (95% confidence interval)	P‡	Mean (95% confidence interval)	P
Vehicle	20	170 (146–193)		7.65 (6.20–9.10)
Day 3 post-injection	18	122 (98–146)	0.017	2.89 (1.94–3.84)	<0.0001
Day 7 post-injection	19	151 (127–176)	NS	4.94 (3.90–5.98)	0.008
Day 14 post-injection	18	177 (153–201)	NS	5.96 (4.69–7.22)	NS

NOTE: 175,000 231-BR cells were injected into the left ventricle of the heart of BALB/c nude mice. Results of two experiments are combined.

Abbreviation: Not significant.

^*Size of metastases was determined by a 16 mm² ocular grid. Large metastases are >300 μm on the longest axis.

^†Mean number of metastases per section counted in 10-step sections from one hemisphere of the brain.

^‡A two-factor factorial ANOVA was used to determine significance. All P values are two-tailed and adjusted for multiple comparisons using Dunnett’s method.