Figure 6.

Maximum-likelihood phylogenetic tree of the members of the order Kiritimatiellales constructed from concatenated alignments of single-copy genes. Two Kiritimatiellae genomes outside of the Kiritimatiellales order (GTDB order UBA8416) were taken as outgroup and were omitted from the figure. Branch support is indicated with SH-like approximate likelihood ratio test values and ultra-fast bootstraps values, in that order. Black circles indicate support values of 100/100. The scale bar indicates substitutions per site. All cultured members are highlighted with bold font. The proposed novel genus and family are shaded in blue and grey, respectively. Since the UBA5540 and the SAT197 metagenome-assembled genomes (MAGs) share only 48% amino acid identity (AAI), we tentatively excluded MAG UBA5540 from the proposed novel family. MAG UBA5540 represents the uncultivated MSBL3 cluster based on the classification of its 16S rRNA gene using Silva ACT [56], and thus may represent an additional novel family within the Kiritimatiellales. NCBI accession numbers are indicated between parentheses, except for TARA oceans MAGs from Delmont, et al. [38] which can be accessed from https://doi.org/10.6084/m9.figshare.4902923.