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. 2020 May 28;12(6):488. doi: 10.3390/pharmaceutics12060488

Figure 2.

Figure 2

Alteration of mitochondrial membrane potential and ROS generation by stigmasterol in both cells. (A,B) JC-1 dye was used to investigate the alteration of mitochondrial membrane potential by stigmasterol (0, 5, 10, and 20 μg/mL). Quadrant of the dot blot represented the state of mitochondrial membrane potential in ES2 and OV90 cells. Comparative graph represented the loss of the mitochondrial membrane potential compared to the control group (100%) in ES2 and OV90 cells. (C,D) DCF fluorescence intensity was observed to investigate the change by stigmasterol treatment (0, 5, 10, and 20 μg/mL). The histogram represented the state of ROS production in ES2 (Figure 2C, left) and OV90 (Figure 2D, left) cells. Comparative graph represented ROS production compared to vehicle-treated control (100%) in ES2 (Figure 2C, right) and OV90 (Figure 2D, right) cells. DCF: dichlorofluorescein.