Figure 2.
Deletion of subtilisin-like gene APASM_4178 led to delayed mycelial fragmentation, increased growth and AP-3 yield. (A) Confocal micrographs of the APASM_4178-deleted mutant WYT-5. Scale bar: 10 μm. Magnification, 2500×; (B) Growth comparison between the wild-type and the mutant WYT-5. Error bars: mean ± SD (n = 3 biological replicates); (C) AP-3 yield comparison between the wild-type and the mutant WYT-5. Error bars: mean ± SD (n = 3 biological replicates); (D) Phase-contrast micrographs of WYT-5 complemented with cloned APASM_4178. Scale: 5 μm. Magnification, 400×.
