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. 2020 Jun 3;10(6):851. doi: 10.3390/biom10060851

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression of subtilisin inhibitor (SSI) genes in A. pretiosum ATCC 31280 alleviated mycelial fragmentation. (A) Confocal micrographs of mutant with SSI gene APASM_3064 overexpressed. Scale bar: 10 μm. Magnification, 2500×; (B) Confocal micrographs of mutant with SSI gene APASM_6209 overexpressed. Scale bar: 10 μm. Magnification, 2500×; (C) Growth comparison between 31280::pLQ856, the wild-type integrated with vector plasmid pLQ856, and mutants with APASM_3064 or APASM_3064 overexpressed. Error bars: mean ± SD (n = 3 biological replicates); (D) Yield comparison between the control strain 31280::pLQ856 and mutants with APASM_3064 or APASM_6209 overexpressed. Error bars: mean ± SD (n = 3 biological replicates).