Congo red staining of the trachea (A–G) and lungs (H–N). (A,H) Healthy control, (B,I) PM only, (C,J) OVA only, (D,K) OVA + PM, (E,L) OVA + PM + Fucoidans (100 mg/Kg), (F,M) OVA + PM + Fucoidans (400 mg/Kg), (G,N) OVA + PM + Prednisone (5 mg/Kg). (O) Number of Congo red stained cells per site in the trachea, (P) Number of Congo red stained cells per site in the lungs. Values are expressed as means ±SEM (n = 3). ** (p < 0.005), *** (p < 0.0005) represent significant increases compared with the healthy control, and ††† (p < 0.0005) represents a significant decrease compared with the OVA + PM group. ## (p < 0.005), ### (p < 0.0005) represents a significant increase compared with the OVA only group. The scale bar of (A–N) is 25 µm.