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. 2020 Jun 15;25(12):2759. doi: 10.3390/molecules25122759

Table 2.

Effect of growth regulators on callus-mass increase from stem and leaf explants. Mean value ± standard error, n = 3.

Treatments Callus Mass Increase mg
After one Month After two Months
Callus from Leaf
MS (−) 0.0 ± 0.0 0.0 ± 0.0
1.0 µM NAA 10.0 ± 0.9 d 19.3 ± 1.9 d
2.0 µM NAA 25.7 ± 1.2 a 39.3 ± 0.1 a
3.0 µM NAA 9.4 ± 0.1 d 36.5 ± 1.0 b
1.0 µM NAA + 1.0 µM BAP 16.1 ± 0.3 c 26.5 ± 1.8 c
2.0 µM NAA + 1.0 µM BAP 18.4 ± 1.0 b 29.7 ± 1.9 bc
3.0 µM NAA + 1.0 µM BAP 15.8 ± 0.8 c 32.1 ± 2.2 b
Average 15.90 30.5
Callus from stem
MS (−) 0.0 ± 0.0 0.0 ± 0.0
1.0 µM NAA 2.8 ± 0.3 ef 6.3 ± 0.5 f
2.0 µM NAA 3.7 ± 0.3 e 8.3 ± 0.1 e
3.0 µM NAA 0.4 ± 0.05 g 2.5 ± 0.1 g
1.0 µM NAA + 1.0 µM BAP 0.6 ± 0.0 g 5.0 ± 0.8 f
2.0 µM NAA +1.0 µM BAP 1.0 ± 0.3 g 6.7 ± 0.3 f
3.0 µM NAA + 1.0 µM BAP 1.8 ± 0.2 fg 6.3 ± 0.2 f
Average 1.71 5.85
p-Value (growth regulator x explant type) <0.00100 <0.00001

Note: Means not sharing a common letter were significantly different in column (p < 0.05); NAA, α –naphthalene acetic acid; BAP, 6-benzylaminopurine.