Table 2.
Treatments | Callus Mass Increase mg | |
---|---|---|
After one Month | After two Months | |
Callus from Leaf | ||
MS (−) | 0.0 ± 0.0 | 0.0 ± 0.0 |
1.0 µM NAA | 10.0 ± 0.9 d | 19.3 ± 1.9 d |
2.0 µM NAA | 25.7 ± 1.2 a | 39.3 ± 0.1 a |
3.0 µM NAA | 9.4 ± 0.1 d | 36.5 ± 1.0 b |
1.0 µM NAA + 1.0 µM BAP | 16.1 ± 0.3 c | 26.5 ± 1.8 c |
2.0 µM NAA + 1.0 µM BAP | 18.4 ± 1.0 b | 29.7 ± 1.9 bc |
3.0 µM NAA + 1.0 µM BAP | 15.8 ± 0.8 c | 32.1 ± 2.2 b |
Average | 15.90 | 30.5 |
Callus from stem | ||
MS (−) | 0.0 ± 0.0 | 0.0 ± 0.0 |
1.0 µM NAA | 2.8 ± 0.3 ef | 6.3 ± 0.5 f |
2.0 µM NAA | 3.7 ± 0.3 e | 8.3 ± 0.1 e |
3.0 µM NAA | 0.4 ± 0.05 g | 2.5 ± 0.1 g |
1.0 µM NAA + 1.0 µM BAP | 0.6 ± 0.0 g | 5.0 ± 0.8 f |
2.0 µM NAA +1.0 µM BAP | 1.0 ± 0.3 g | 6.7 ± 0.3 f |
3.0 µM NAA + 1.0 µM BAP | 1.8 ± 0.2 fg | 6.3 ± 0.2 f |
Average | 1.71 | 5.85 |
p-Value (growth regulator x explant type) | <0.00100 | <0.00001 |
Note: Means not sharing a common letter were significantly different in column (p < 0.05); NAA, α –naphthalene acetic acid; BAP, 6-benzylaminopurine.