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. 2020 Jun 4;9(6):716. doi: 10.3390/plants9060716

Table 1.

Chemical profiles of Essential oils (EOs)of Teucrium polium and Thymus decussatus extracted using hydrodistillation (HD) and microwave-assisted extraction (MAE) methods.

No. Compound Rt KIExp KILit T. polium T. decussatus Identification b
HD MAE HD MAE
Monoterpene hydrocarbons
1 α-Thujene 4.57 925 925 0.52 ± 0.01 a 1.26 ± 0.03 0.04 ± 0.01 ---- MS, KI
2 α-Pinene 4.77 935 936 8.94 ± 0.06 11.58 ± 0.09 ---- ---- MS, KI
3 Camphene 5.80 954 954 ---- 0.44 ± 0.02 ---- 0.30 ± 0.02 MS, KI
4 2,4(10)-Thujadiene 5.96 961 960 ---- 0.32 ± 0.01 ---- ---- MS, KI
5 Sabinene 7.44 975 975 2.94 ± 0.04 1.14 ± 0.03 ---- ---- MS, KI
6 β-Pinene 7.54 978 979 7.38 ± 0.05 8.20 ± 0.09 0.09 ± 0.01 0.54 ± 0.03 MS, KI
7 α-Myrcene 10.19 986 986 2.90 ± 0.04 ---- 0.16 ± 0.01 1.04 ± 0.03 MS, KI
8 p-Cymene 10.20 1024 1026 ---- ---- 3.61 ± 0.04 16.98 ± 0.10 MS, KI
9 Limonene 10.94 1034 1033 1.10 ± 0.02 0.58 ± 0.03 ---- ---- MS, KI
10 α-Terpinene 12.18 1018 1018 ---- ---- ---- 1.01 ± 0.03 MS, KI
11 β-Terpinene 12.23 1031 1032 ---- ---- 0.23 ± 0.01 ---- MS, KI
12 β-Phellandrene 12.64 1045 1044 ---- ---- ---- 0.21 ± 0.01 MS, KI
13 γ-Terpinene 14.43 1061 1061 0.34 ± 0.02 ---- 0.49 ± 0.01 2.51 ± 0.06 MS, KI
14 β-Terpinolene 15.15 1084 1086 ---- ---- 0.12 ± 0.01 0.34 ± 0.01 MS, KI
Oxygenated monoterpenes
15 α-Fenchyl alcohol 12.70 1106 1107 0.39 ± 0.02 ---- ---- ---- MS, KI
16 cis-Verbenol 13.65 1142 1144 0.34 ± 0.03 ---- ---- ---- MS, KI
17 Menthol 14.17 1181 1182 ---- ---- ---- 0.54 ± 0.01 MS, KI
18 α-Terpineol 20.28 1189 1189 0.44 ± 0.03 ---- ---- ---- MS, KI
19 Thymol methyl ether 20.41 1236 1237 ---- ---- ---- 0.03 ± 0.01 MS, KI
20 Dihydrocarvone 21.31 1243 1242 ---- ---- ---- 0.06 ± 0.01 MS, KI
21 Carvacrol 22.79 1304 1304 ---- ---- 94.40 ± 0.52 75.91 ± 0.61 MS, KI
22 α-Terpinenyl acetate 25.21 1349 1350 0.51 ± 0.02 ---- ---- ---- MS, KI
23 Thymyl acetate 28.55 1357 1357 ---- ---- 0.05 ± 0.01 0.06 ± 0.01 MS, KI
Sesquiterpene hydrocarbons
24 α-Cubebene 21.37 1351 1351 ---- 1.10 ± 0.03 ---- ---- MS, KI
25 α-Bourbonene 25.43 1374 1374 ---- 0.48 ± 0.04 ---- ---- MS, KI
26 α-Copaene 25.77 1377 1376 0.47 ± 0.02 1.34 ± 0.04 ---- ---- MS, KI
27 β-Elemene 26.52 1385 1384 ---- 0.42 ± 0.03 ---- ---- MS, KI
28 Longifolene 27.61 1401 1402 ---- ---- 0.07 ± 0.01 ---- MS, KI
29 cis-Caryophyllene 27.89 1410 1409 0.97 ± 0.03 1.72 ± 0.05 0.33 ± 0.02 0.17 ± 0.01 MS, KI
30 Clovene 29.37 1426 1425 ---- ---- 0.13 ± 0.01 ---- MS, KI
31 Aromadendrene 29.55 1440 1439 3.86 ± 0.04 0.33 ± 0.03 0.10 ± 0.01 0.04 ± 0.01 MS, KI
32 β-Neoclovene 30.56 1455 1454 ---- ---- 0.04 ± 0.01 ---- MS, KI
33 α-Gurjunene 30.74 1475 1475 0.68 ± 0.03 ---- ---- ---- MS, KI
34 Germacrene-D 31.99 1480 1480 2.21 ± 0.06 7.25 ± 0.46 ---- ---- MS, KI
35 β-Selinene 33.44 1485 1485 ---- ---- 0.04 ± 0.01 ---- MS, KI
36 Guaia-1(10),11-diene 33.72 1488 1488 ---- 1.69 ± 0.07 ---- ---- MS, KI
37 β-Muurolene 34.08 1493 1493 0.57 ± 0.04 5.55 ± 0.06 ---- 0.05 ± 0.01 MS, KI
38 α-Muurolene 34.54 1498 1499 0.72 ± 0.01 7.64 ± 0.58 ---- ---- MS, KI
39 δ-Cadinene 35.03 1525 1524 7.04 ± 0.31 25.13 ± 0.43 0.06 ± 0.01 0.08 ± 0.01 MS, KI
40 Cadina-1(10),4-diene 35.89 1530 1530 ---- 1.83 ± 0.05 ---- ---- MS, KI
Oxygenated sesquiterpenes
41 Spathulenol 28.61 1515 1516 ---- 0.51 ± 0.02 ---- ---- MS, KI
42 1,5-Epoxy-salvial-4(14)-ene 29.64 1548 1548 0.86 ± 0.02 0.78 ± 0.03 ---- ---- MS, KI
43 Ledol 29.67 1564 1565 0.69 ± 0.04 0.58 ± 0.03 ---- ---- MS, KI
44 4-epi-Cubebol 29.29 1572 1574 ---- 0.30 ± 0.01 ---- ---- MS, KI
45 Germacrene D-4-ol 30.71 1578 1577 5.65 ± 0.31 ---- ---- ---- MS, KI
46 Τ-Cadinol 32.23 1638 1640 2.06 ± 0.05 7.61 ± 0.12 ---- ---- MS, KI
47 Cubenol 32.31 1641 1642 ---- 0.35 ± 0.01 ---- ---- MS, KI
48 Τ-Muurolol 32.74 1646 1646 12.88 ± 0.15 ---- ---- ---- MS, KI
49 α-Cadinol 34.16 1654 1653 2.01 ± 0.03 5.49 ± 0.10 ---- ---- MS, KI
50 Alloaromadendrene oxide 39.89 1672 1672 0.53 ± 0.01 6.09 ± 0.23 ---- ---- MS, KI
51 6-Epi-shyobunol 41.92 1682 1680 33.00 ± 0.72 0.29 ± 0.01 ---- ---- MS, KI
Non-oxygenated hydrocarbons
52 Nonadecane 45.04 1900 1900 ---- ---- 0.03 ± 0.01 0.03 ± 0.01 MS, KI
53 Heptacosane 51.54 2700 2700 ---- ---- 0.01 ± 0.00 0.10 ± 0.02 MS, KI
Monoterpene hydrocarbons 24.12 23.52 4.74 22.93
Oxygenated monoterpenes 1.68 0 94.45 76.6
Sesquiterpene hydrocarbons 16.52 54.48 0.73 0.34
Oxygenated sesquiterpenes 57.68 22 0 0
Non-oxygenated hydrocarbons 0 0 0.04 0.13
Total 100% 100% 100% 100%

Rt: retention time, KILit: published Kovats retention indices; KIexp: Kovats index determined experimentally relative to C8–C28 n-alkanes; a Values are mean ± standard deviation; b the identification of EO constituents based on the comparison of the mass spectral data and Kovats indices (KI) with those of NIST Mass Spectral Library (2011) and Wiley Registry of Mass Spectral Data 8th edition and literature. HD: hydrodistillation, MAE: microwave-assisted extraction.