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Flow cytometry analysis using Annexin V-FITC/propidium iodide (PI) double staining of human melanoma A375 cells incubated with 3-O-acetylursolic acid (UAA) and ursolic acid (UA) (GI50, 12–24 h). (A) Quantification of unaffected, early/late apoptotic, and necrotic populations. (B) Apoptotic phases in control and treated cells. Data are presented as the mean ± SD (n = 3), (*) p < 0.05; (**) p < 0.01; (***) p < 0.001.
