Figure 5.

Effects of Cd on the endocytosis efficiencies of β₂-MG and MT into S1 and S2 cells. S1 and S2 cells were exposed to CdCl₂ for 1, 3, and 6 days and then incubated with FITC-β₂-MG (A) or FITC-MT (B) for 30 min. The endocytosis efficiencies were determined by flow cytometry and expressed as percentages of the control cells (no exposure to Cd). Open and closed columns represent S1 and S2 cells, respectively. Data are presented as means ± SD (n = 3–4). Statistical significance of the dose dependence determined by one-way ANOVA was detected in the following settings: day1-S1 cells (p < 0.05), day1-S2 cells (p < 0.05), day3-S1 cells (p < 0.05), and day3-S2 cells (p < 0.01) for β₂-MG (A), and day3-S1 cells (p < 0.05) and day3-S2 cells (p < 0.05) for MT (B). Statistical significances compared with the control cells determined by Bonferroni multiple comparisons are indicated as * p < 0.05, ** p < 0.01 (S1 cells) and # p < 0.05, ## p < 0.01 (S2 cells).