Figure 4.
Fate of carbon from glucose in cultivations of C. glutamicum ARO strains carrying the empty vector (left) or pEKEx3-trpEFBR (right). Carbon (given in mol%) derived from glucose found after 48 h in secreted products anthranilate (blue), shikimate (maroon), α-ketoglutarate (light red), trehalose (green), lactate (black) as well as in the formed biomass (yellow) are shown for C. glutamicum strains C1*, ARO03, ARO07, and ARO09 harboring either pEKEx3 (left) or pEKEx3-trpEFBRfbr (right). Carbon that could not be accounted for is depicted in gray (other). Values were determined from duplicate cultures. Experimental error was less than 20%. Abbreviations used: α-KG, α-ketoglutarate. Carbon distribution of all ARO strains can be found in the Supplementary Data (Figure S1; Figure S2).