Table 2.
Oligonucleotides used in this study.
| Name | Oligonucleotide Sequence (5′ to 3′) |
|---|---|
| vdh-conf-fw | GACCTCTAGGGCAGCAGTG |
| vdh-conf-rv | CTGTTCAGCGGATTAGCG |
| ldhA-conf-fw | TGATGGCACCAGTTGCGATGT |
| ldhA-conf-rv | CCATGATGCAGGATGGAGTA |
| sugR-conf-fw | CGAGATGCTGTGGTTTTGAG |
| sugR-conf-rv | GCTTATCGGGTGTGGGAATG |
| US-aroR-fw | CCTGCAGGTCGACTCTAGAGCGATGCAGAATAATGCAGTTAG |
| US-aroR-rv | CGGAGCTTGCCTGGGAGTTTGGAACCTTAACACACTTTC |
| PilvC-aroR-fw | GAAAGTGTGTTAAGGTTCCAAACTCCCAGGCAAGCTCCGCGC |
| PilvC-aroR-rv | GAAAAAACCTCCTTTAGTGTGTAGTTAAGTTATGGTGATGGGAGAAAATCTCGCCTTTCG |
| DS-aroR-fw | ATCACCATAACTTAACTACACACTAAAGGAGGTTTTTTCATGAGTTCTCCAGTCTCACTCGAAAAC |
| DS-aroR-rv | GAATTCGAGCTCGGTACCCGGGCAATGCGCAAGCCCTCTGGG |
| aroR-conf-fw | GGAACTCCCGTTGAGGTG |
| aroR-conf-rv | GTGGTACGAGCGCCGATTG |
| US-qsuA-fw | CCTGCAGGTCGACTCTAGAGGTTGGCAGCGCAACCAGTC |
| US-qsuA-rv | CTACTGACACGCTAAAACGCTGTCGATCCTGTTCATCG |
| Ptuf-qsuC-fw | CGATGAACAGGATCGACAGCGTTTTAGCGTGTCAGTAG |
| Ptuf-qsuC-rv | CTGAAGGGCCTCCTTTC TCCTCCTGGACTTCGTGG |
| qsuC-fw | GGAGAAAGGAGGCCCTTCAGATGCCTGGAAAAATTCTCCTCC |
| qsuC-rv | GTCGAGGTTTTACTGACTCTTCTACTTTTTGAGATTTGCCAGG |
| DS-qsuD-fw | CTCAAAAAGTAGAAGAGTCAGTAAAACCTCGACGC |
| DS-qsuD-rv | GAATTCGAGCTCGGTACCCGGGATTTCGCGGATGGGTCTAAGTATG |
| qsu-conf-fw | GTTCGTGGACAAGTGTGGTGG |
| qsu-conf-rv | GTTCGTGGACAAGTGTGGTGG |
| US-ppc-fw | GCCTGCAGGTCGACTCTAGAGCGCTCAGGAAGTGTGCAAGGC |
| US-ppc-rv | GTACTACCCAGCCGGCTGGGGATCCCTACTTTAAACACTCTTTCACATTGAGGGTG |
| Psod-aroB-fw | AATGTGAAAGAGTGTTTAAAGTAGGAAGCGCCTCATCAGCGGTAAC |
| Psod-aroB-rv | CTCCTTTAAAAATAAGTCGCCTACC AAAATCCTTTCGTAGGTTTCCGC |
| aroB-fw | GCGGAAACCTACGAAAGGATTTTGGTAGGCGACTTATTTTTAAAGGAGGTTTTTTATGAGCGCAGTGCAGATTTTC |
| aroB-rv | CTTCTCTCATCCGCCAAAATTAGTGGCTGATTGCCTCATAAG |
| Term-aroB-fw | CTTATGAGGCAATCAGCCACTAATTTTGGCGGATGAGAGAAG |
| Term-aroB-rv | AGTACTACCCAGCCGGCTGGGGATCCAAAAGAGTTTGTAGAAACGC |
| DS-ppc-fw | TGAAAGAGTGTTTAAAGTAGGGATCCCCAGCCGGCTGGGTAGTAC |
| DS-ppc-rv | GAATTCGAGCTCGGTACCCGGGCAGTGGGGAGACAACAGGTCG |
| ppc-conf-fw | CCGTCGGGAAACAGTTCCCC |
| ppc-conf-rv | GCAGACCCGTAAGTCCCTTGC |
| US-tkt-fw | GCATGCCTGCAGGTCGACTCTAGAGTGACCCAGGTGGACGCCAAC |
| US-tkt-rv | GTGGACATTCGCAGGGTAACGGCCAAGGTGTGATCAATCTTAAGTC |
| Ptuf-tkt-fw | GACTTAAGATTGATCACACCTTGGCCGTTACCCTGCGAATGTCCAC |
| Ptuf-tkt-rv | CGTCAAGGTGGTCATCTGAAGGGCCTCCTTTCTGTATGTCCTCCTGGACTTC |
| DS-tkt-fw | CAGGAGGACATACAGAAAGGAGGCCCTTCAGATGACCACCTTGACGCTGTC |
| DS-tkt-rv | GAATTCGAGCTCGGTACCCGGGTGGCGGTACTCAGGGTGTCC |
| tkt-conf-fw | GTTCCCGAATCAATCTTTTTAATG |
| tkt-conf-rv | GACCCTGGCCAAGAGGGCCAGTG |
| US-iolR-fw | GCCTGCAGGTCGACTCTAGAGCGACCCTCACGATCGCATG |
| US-iolR-rv | CTACTGACACGCTAAAACGCGATGTCTCCTTTCGTTGCCC |
| Ptuf-aroE-fw | GGGCAACGAAAGGAGACATCGCGTTTTAGCGTGTCAGTAG |
| Ptuf-aroE-rv | CCCATCTGAAGGGCCTCCTTTCTCCTCCTGGACTTCGTGGTG |
| aroE-fw | GGAGAAAGGAGGCCCTTCAGATGGGTTCTCACATCACTCACCG |
| aroE-rv | CAGAAGGGCTCTTTGGTTTATTTCTTAGTGTTCTTCTGAGATGCCTAAAGACTC |
| DS-iolR-fw | GAGTCTTTAGGCATCTCAGAAGAACACTAAGAAATAAACCAAAGAGCCCTTCTG |
| DS-iolR-rv | GAATTCGAGCTCGGTACCCGGGCGCTCTCCATCCGCTGGAC |
| iolR-conf-fw | CAGATAGAGGAACCCAAGGCG |
| iolR-conf-rv | GGACTTCGTGAGTGCTCGTC |
| sugR_reintegr-fw | CTGCAGGTCGACTCTAGAGCCTGCGCAGGGACCCTAATAAG |
| sugR_reintegr-rv | GAATTCGAGCTCGGTACCCGGGCCTGCAGTAAAAGATTCCCGC |
| x3-trpE-fw | CCTGCAGGTCGACTCTAGAGGAAAGGAGGCCCTTCAGATGCAAACACAAAAACCGACTCTCGAACTG |
| x3-trpE-rv | AAAACGACGGCCAGTGAATTTCAGAAAGTCTCCTGTGCATGATGCGC |
| pGANMT-sahH-fw | ATGAGCTCGGTACCCGGGCGGGACGAAGAGAACCGTTACAAGAATAAAGGAGGTTTTTTATGGCACAGGTTATGGACTTC |
| pGANMT-sahH-rv | CTGCAGGTCGACTCTAGAGTTAGTAGCGGTAGTGCTCCGG |
Ribosomal binding sites are in bold, and binding regions of Gibson oligonucleotides are underlined.