Table 3.

Contents of residual DNA, total collagen and glycosaminoglycan (GAG) of porcine skin following decellularization at various concentrations of formic acid (FA) for different time points compared to fresh porcine skin treated with phosphate-buffered saline (PBS) under the same processing steps. Values are presented as the mean ± standard deviation (n = 3). (* p < 0.05 compared to fresh porcine skin treated with PBS).

Treatments	0 h	24 h	48 h	72 h
Residual DNA (ng/mg dry weight)
PBS	349.77 ± 8.33	-	-	-
10% FA	-	93.43 ± 1.14 *	90.89 ± 2.02 *	87.16 ± 2.86 *
20% FA	-	87.84 ± 0.85 *	87.35 ± 3.90 *	77.32 ± 0.82 *
30% FA	-	67.01 ± 3.75 *	58.87 ± 0.51 *	42.95 ± 0.73 *
Total collagen (µg/mg dry weight)
PBS	715.39 ± 7.34	-	-	-
10% FA	-	647.15 ± 18.51	630.45 ± 13.43	625.87 ± 2.38
20% FA	-	610.25 ± 2.13	583.85 ± 3.90	566.52 ± 7.34
30% FA	-	574.69 ± 7.46	567.24 ± 7.97	556.01 ± 5.94
Glycosaminoglycan (µg/mg dry weight)
PBS	14.88 ± 1.21	-	-	-
10% FA	-	10.61 ± 0.32 *	8.97 ± 0.40 *	9.59 ± 0.32 *
20% FA	-	9.19 ± 0.08 *	7.79 ± 0.08 *	6.52 ± 0.32 *
30% FA	-	7.09 ± 0.64 *	6.86 ± 0.80 *	5.50 ± 0.16 *