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. 2020 May 30;8(6):824. doi: 10.3390/microorganisms8060824

Figure 3.

Figure 3

DCs primed by secretomes of HMPV-infected L-HMVECs induce Th2 polarized cells. Purified CD4+ T cells were co-cultured with allogenic DCs pretreated with mock or HMPV-infected L-HMVEC secretomes harvested at 21 days PI. After 7 days cells were treated with PMA, Ionomycin, and Brefeldine, then fixed, permeabilized, stained for IFN-γ and IL-4 and analysed in flow cytometry as described in Section 2. Cell gating was on CD4+ T cell population. In the left panel intracellular cytokine expression is represented as dot-plots. Numbers indicate the % of positive cells in each quadrant. Data represent one of 10 independent experiments performed with cells from different donors. Box-plot graph (right panel) represents % of IL-4-producing cells generated after the allogenic culture of naïve CD4 T cells with DCs treated with mock or HMPV-infected L-HMVEC secretomes. Lower quartile, median, upper quartile, and largest observations are represented. * p < 0.05.