Figure 4.
Role of P-selectin in the adhesion of platelets or MEG-01 cells. (a) Adhesion of platelets (green) was quantified following stimulation with 1, 3, and 10 μM Aβ1-40 for 1 h with or without 1 μg/mL anti-P-selectin monoclonal antibody pre-treated for 30 min on fibronectin-coated coverslips. Data are mean ± SEM of five experiments. (b) Adhesion of MEG-01 cells (top, green fluorescence; bottom, phase-contrast image) was quantified following stimulation with 1, 3, and 10 μM Aβ1-40 for 1 h with or without 1 μg/mL anti-P-selectin monoclonal antibody pre-treated for 30 min on fibronectin-coated coverslips. (insert) Arrowhead indicates spreading of MEG-01 cells. Data are mean ± SEM of six experiments.