Table 1.

Summary of the SWOT (Strengths–Weaknesses–Opportunities–Threats) Analysis of Different Techniques for EV Detection and Quantification.

	Strengths	Weaknesses	Opportunities	Threats
ELISA	Flexibility Commercial kits	Multistep Time consuming	Detection of EV from different sources Affordable Automation available	Development of biosensors Multiple-targeted detection systems
LFIA	Cost-effective on-site detection	Lack of sensitivity	Potential portable detectors	Development of more robust systems
NTA	Measurement and quantification	Not affordable/available for research groups	Phenotyping of EV sub-populations Applications in drug delivery systems	AF4 enables EV fractionation
AF4	Size fractionation High reproducibility	Requires skilled operator	Potential clinical and pharmaceutical applications	Eluted fractions may not be used for further functional studies