Effects of sevelamer on hepatic steatosis in choline-deficient, L-amino acid-defined, high-fat (CDHF)-fed mice. (A) Experimental protocols. (B) Changes in body weights during experimental period. (C) Liver weight at the end of experiment. (D) Serum levels of alanine aminotransferase (ALT) and triglyceride (TG). (E) Representative microphotographs of liver sections stained with hematoxylin–eosin (H&E) in the experimental groups. Scale Bar; 50 µm. Histological score for steatosis, inflammation and ballooning according to nonalcoholic fatty liver disease (NAFLD) Activity Score. (F) Serum levels of phosphorus and calcium. Vehicle (veh), sevelamer (sev), choline-sufficient amino acid containing normal fat (CSNF), choline-deficient, L-amino acid-defined containing high-fat (CDHF), not detected (ND). Data are mean ± SD (n = 10). * p < 0.05, indicating a significant difference between groups. a
p < 0.05, indicating a significant difference compared with CSNF + veh group. b
p < 0.05, indicating a significant difference compared with CSNF + sev group.