Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 10;176(1):236–252. doi: 10.1093/toxsci/kfaa048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society of Toxicology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 4. — A, The ToxTracker toxicity pathway markers Bscl2-GFP and Rtkn-GFP (DNA damage), Btg2-GFP (p53-associated cellular stress), Srxn1-GFP and Blvrb-GFP (oxidative stress), and Ddit-GFP (unfolded protein response) observed by flow cytometry after 24 h exposure to coumarin (0, 62.5, 125, 250, 500, and 1000 μM) in mES cells. A 2-fold green fluorescent protein (GFP) induction level was defined as the threshold for a positive test result, whilst the cell survival rate determined by cell count was always > 25%. Each data point on the graph represents the mean fold induction of 3 independent experiments ± SD. B, Summarized cell survival is expressed as the average across all cell lines and the 3 independent experiments ± SD per concentration. .