Table 5.

PoD_T Values (µM) for Coumarin Treated Across 4 Cell Models for 24 h Using a Subset of Proposed Approaches for Gene Selection Based on Those Proposed by Farmahin et al. (2017)

Cell Model	HepG2	MCF7	HepaRG 2D	HepaRG 3D
Pathway-level tests PoDT (µM)	(308 pathways)	(0 pathways)	(17 pathways)	(2 pathways)
20 pathways with the lowest p value Reactome	70	NA	58*	46*
20 pathways with the lowest BMD Reactome	44	NA	58*	46*
BMD of Reactome pathway with lowest BMD that meets significance threshold criteria	31	NA	38	41
Gene-level tests PoDT (µM)	(1570 genes)	(47 genes)	(87 genes)	(9 genes)
Mean BMD of 20 genes with largest fold change	6	3	54	55
Mean BMD of genes between 25th and 75th percentile	17	1	59	46*

Highlighted (*) are values where the number of pathways or genes was below the recommended number (ie, 20) for grouping. Abbreviation: NA, not applicable.