Figure 5.

Arsenic effects on EGFR and mitochondrial regulation of nuclear cyclinD1. A, Representative images of cyclin D1 immunofluorescence in undifferentiated RC replated in arsenic-medium. AG-1478 was added simultaneously with arsenic in the differentiation protocol. B–D, Quantitative comparison of nuclear cyclin D1 levels in replated RC. Groups of cells in (C) and (D) were treated with SS-31 or XJB-5-131 after replating and all groups in (B–D) were fixed and analyzed after 3 days in arsenic-free culture. Representative images of cells from (C) and (D) are provided in Supplementary Figure 2. All experiments were repeated 3 times and the data are presented as mean ± SEM of relative fluorescence per cell captured in 5 separate images. Group comparisons were made using ANOVA followed by Tukey’s post hoc test for significance (*p < .05, **p < .01 relative to control, ^p < .05, ^^p < .01 relative to arsenic).