Table 1.

Initial Uptake Kinetics of Conjugated, Primary Bile Acids in OSTab Cells

Conjugated Bile Acid	Initial Uptake Rate ± SD (pmol/min/mg Protein)	y-Intercept ± SD (pmol/mg Protein)
TCDCA	216.2 ± 10.0	26.2 ± 2.9
GCDCA	111.8 ± 8.2	23.7 ± 2.4
TCA	35.3 ± 0.5	5.8 ± 0.2
GCA	62.7 ± 3.0	6.4 ± 0.9

The initial uptake rate (slope) and y-intercept of conjugated bile acid species (5 µM) in OSTab cells were determined by performing linear regression on the initial uptake over the first 30 s (Figure 1).

Abbreviations: GCA, glycocholate; GCDCA, glycochenodeoxycholate; OSTab cells, OSTα/β-overexpressing cells; TCA, taurocholate; TCDCA, taurochenodeoxycholate.