Figure 1.

Diesel exhaust particulate extract toxicity in ZF. Dechorionated embryos were exposed to 20 µg/ml DEPe in 0.01% DMSO from 24 to 72 hpf and imaged for gross morphological defects in both the heart (left) as well as the tail (right) (A). Square, clear-walled 96-well plates were used for behavioral analysis in 7 dpf embryos treated with 25 µg/ml DEPe from 24 to 48 hpf and washed out. Light cycling was analyzed averaging distance moved per 10 min in both light and dark conditions (dark conditions are shaded in the graph) for 3 cycles with 9–11 larvae per condition (B). Statistical analysis by two-way ANOVA with repeated measures (*p < .05 and SEM).