Table 1. Sequence of siRNAsa and Melting Temperatures (Tm).
| siRNA | siRNA sequence | Tm (° C) |
|---|---|---|
| wt | 5′-CUUACGCUGAGUACUUCGAdTdT-3′ | 74.1 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 | ||
| 1 | 5′-CdUxdUACGCUGAGUACdUxdUCGAdTdT-3′ | 71.8 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 | ||
| 2 | 5′ - CUUACGCUGAGUACUUCGAdUxdU - 3′ | 74.4 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 | ||
| 3 | 5′-CUUACGCUGAGUACUUCGAdUxdUdT-3′ | 77.1 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 | ||
| 4 | 5′-CUUACGCUGAGUACUUCGAdTxdT-3′ | 73.4 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 | ||
| 5 | 5′-CUUACGCUGAGUACUUCGAdTxdTdT-3′ | 75.1 |
| 3′-dTdTGAAUGCGACUCAUGAAGCU-5 |
a
dTxdT corresponds to the deoxythymidine-based modification; dUxdU corresponds to the deoxyuridine-based modification. The top strand is the passenger strand; the bottom strand is the guide strand. The guide strand is 5′-phosphorylated.