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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: J Immunol. 2020 Feb 7;204(6):1508–1520. doi: 10.4049/jimmunol.1901120

Figure 1. BAFF-RFP detection in blood and splenic cell subsets.

Figure 1.

A. BAFF-RFP by flow cytometry in BM Nph and a subset of B220+ B cells in BAFF-RFP+/− mice (See Methods for gating strategy). B. BAFF mRNA by qPCR in sorted Nphs, B220+ B cells from WT mice, B220+RFP+ B cells and B220+RFP− B cells from BAFF-RFP+/− mice. Baff mRNA expression in arbitrary units relative to 18S. A and B, data show one representative of two experiments. C and D, expression of BAFF-RFP in PBMC (C) and splenic (D, E and F) cell populations from naïve BAFF-RFP+/− mice. C and D, data show one representative of two (C) or more than five (D, E and F) experiments. In A, C and D, WT (C57BL/6): grey filled histograms; BAFF-RFP+/− mice: black empty histograms. E and F, dot plots of BAFF-RFP expression in myeloid (E), B cells and T cells (F). In A-E, Neutrophils, Nph; Ly6Chi monocytes, Ly6Chi MO; dendritic cells, DC, conventional DC, cDC. For gating strategy of cell populations in Spleen (D, E and F) see Fig. S2B; and for cell populations in BM (A) and PBMCs (C) see Methods PBMCs (C) see Methods.