Table 1.
Effects of sensor type and concentration
| TAS2R8 | TAS2R14 | |||
|---|---|---|---|---|
| Lowest detectiona | Response at 250 µMb | Lowest detection | Response at 250 µMb | |
| YC3.6 8 ng/µL | 64 µM | 1.054 | 250/64 µM | 1.031 |
| YC3.6 16 ng/µL | 250/1000 µM | 1.034 | 250/1000 µM | 1.030 |
| YC3.6 25 ng/µL | 1000 µM | 1.018 | 1000/250 µM | 1.021 |
| Nano140 8 ng/µL | 64 µM | 1.146 | 250/64 µM | 1.047 |
| Nano140 16ng/µL | 64/250 µM | 1.100 | 64 µM | 1.061 |
| Nano140 25ng/µL | 250/64 µM | 1.051 | 64 µM | 1.045 |
| Twitch2B 8 ng/µL | 16/64 µM | 1.203 | 64 µM | 1.093 |
| Twitch2B 16 ng/µL | 64 µM | 1.122 | 16 µM | 1.112 |
| Twitch2B 25 ng/µL | 64 µM | 1.087 | 64 µM | 1.086 |
The most optimal and least optimal conditions are marked in bold.
aDetection threshold of agonist that gives a significant response in arrays 1 and 2 (see Supplementary Figure 1). When thresholds are different, both values of arrays 1 and 2 are given.
bResponse estimate of array 1 for exposure to 250 µM agonist.