Table 2.
Primers used for RT-PCR
| Gene | Accession number | Primers (5′–3′) | Prod (bp) |
|---|---|---|---|
| TRPM5 | NM_0014555 | TGGTAGAGCGCATGATGAAG | 300 |
| ACCAACAGGAAGGTGACCAG | |||
| GNAT3 | NM_001102386 | TCTGGGTATGTGCCAAATGA | 366 |
| GGCCCAGTGTATTCTGGAAA | |||
| PLCB2 | NM_004573 | GTCACCTGAAGGCATGGTCT | 332 |
| TTAAAGGCGCTTTCTGCAAT | |||
| TAS2R1 | NM_019599 | GCATCATTGTGGTGGTGAATG | 632 |
| GGATCAGGAAGGACAGGATAGA | |||
| TAS2R3 | NM_016943 | GAGTTTCTAGGGTGATGGTATGG | 245 |
| GGGAGAAGATGAGCAAAGAGTAG | |||
| TAS2R4 | NM_016944 | ACTCGAGCAGTGTCTGGTTTGTGA | 473 |
| ACTGGACCAGGGTAGCAACTGAAT | |||
| TAS2R5 | NM_018980 | CCATCACCTCCAAGACTTATCC | 254 |
| ATGGAAAGCAGCTCCCTTATAG | |||
| TAS2R7 | NM_023919 | CATGGACTGGGTCAAGAAGAG | 352 |
| CAGTGGCTGGAAGGCTAATAA | |||
| TAS2R8 | NM_023918 | GTGGATTACCACCTGCCTTAAT | 522 |
| CTCTCCAAACTCCACAGCTAAC | |||
| TAS2R9 | NM_023917 | GGCTTCTTTATGCTGCTCTTTC | 442 |
| CGAATCTGCTTGGTGTGTCTA | |||
| TAS2R10 | NM_023921 | ATTCTCACCGGCTTAGCTATTT | 356 |
| GAGATCCCAGACTGTGTCATTC | |||
| TAS2R13 | NM_023920 | GAGCTGTCCTCAGTCGATAAAC | 547 |
| GGGTCTCTGTGTCCTTTGTAAT | |||
| TAS2R14 | NM_023922 | TGCTGCTTCTTGTGACTTCGGTCT | 249 |
| GTGTGCTGCATCTTCTTGCGATGT | |||
| TAS2R16 | NM_016945 | GGTCCCTTGCCGTCTTATTT | 199 |
| GGCCTAGCACTTTCCCTTTAG | |||
| TAS2R38 | NM_176817 | CTGCTGTTCCTGAGTGCTATC | 300 |
| CTGTGAAGTGAGGTCTGCTAAA | |||
| TAS2R39 | NM_176881 | CTGTGGCTGTCCGTGTTTAT | 242 |
| CATGTGTAGGGTGTGTCTCTTG | |||
| TAS2R40 | NM_176882 | GCCTGGCTCAAAGTCTTCTATT | 344 |
| CATGTGTAGGGTGTGTCTCTTG | |||
| TAS2R41 | NM_176883 | CCGACAGTTCTTCCATCTACAC | 316 |
| CCAGTTTCAGGGATGGGAAATA | |||
| TAS2R42 | NM_181429 | GACTGGTAAACTGCTCTGAAGG | 213 |
| AGCCAGGTTGTCAAGTGATTAG | |||
| TAS2R43 | NM_176884 | CCTTTCACCCAGTACCTCATTT | 348 |
| TAS2R44/31 | NM_176885 | CCAGTGTGGTAGTGGTTCTATTT | 245 |
| CGGTTACTGCCCAGACATTAT | |||
| TAS2R45 | NM_176886 | CCAGTACCAAGGTCCACATAAA | 210 |
| GTCTGCTTTAGCTTCTTGTTTCC | |||
| TAS2R46 | NM_176887 | AGATCCCAGCATGAAGGTCCACAT | 255 |
| TTTCACCCAGTACCTCACATGCCA | |||
| TAS2R47/30 | NM_001097643 | TGGTTTGCTCTGGGTGTTATT | 663 |
| GAATGGGTGGGTTGAAGGATAG | |||
| TAS2R48/19 | NM_176888 | TCTAGCAAACCTCATACCCTTTAC | 227 |
| CTCTGCTGTGTCCTAAGATTCC | |||
| TAS2R49/20 | NM_176889 | AGTTGGTTTGCTCTGGGTAATA | 411 |
| CAGGGTCAGAGTGAATGGTATC | |||
| TAS2R50 | NM_176890 | CTGGGTTGTAACCAACCATTTC | 208 |
| CTTCTGCCCACATACTCTCATC | |||
| TAS2R60 | NM_177437 | CCTGGCTCAGTGTCTTCTATTG | 513 |
| GACTCCCACACCCAGAATTT |