Table 4.
Lethal mortality response (LC50) of Lygus lineolaris bioassayed using glass vial and diet cup with the insecticide permethrin at different concentrations
| Bioassay (B)-No. | Concentration response (μg per vial or diet cup) | |||||||
|---|---|---|---|---|---|---|---|---|
| n | Slope ± SE | LC50 (95% CI)a | Probit trend | RR50 (95% CI)d | ||||
| Test for slopeb | Test for GoFc | |||||||
| Χ 2 | P > Χ 2 | Χ 2 | P > Χ 2 | |||||
| B1-Diet | 180 | 0.83 ± 0.19 | 6.99 (2.90–10.97) | 19.31 | <0.0001 | 1.19 | 0.3120 | 2.35 (0.94–5.88) |
| B2-Diet | 180 | 0.79 ± 0.28 | 7.45 (-) | 8.02 | 0.0046 | 2.39 | 0.0664 | 2.50 (0.8–7.85) |
| B3-Diet | 180 | 0.65 ± 0.13 | 3.90 (1.22–7.5) | 25.20 | <0.0001 | 0.69 | 0.5585 | 1.31(0.44–3.89) |
| B4-Diet | 180 | 0.84 ± 0.33 | 6.57 (-) | 6.59 | 0.0103 | 4.5 | 0.0037 | 2.21 (0.64–7.64) |
| B5-Diet | 180 | 0.76 ± 0.15 | 5.88 (2.67–9.5) | 26.44 | <0.0001 | 1.5 | 0.2126 | 1.98 (0.79–0.4.96) |
| B6-Diet | 180 | 1.19 ± 0.288 | 5.35 (2.94–7.38) | 17.20 | <0.0001 | 0.71 | 0.5430 | 1.37 (0.55–3.4) |
| B7-Diet | 180 | 0.68 ± 0.13 | 2.97 (1.14–5.38) | 28.45 | <0.0001 | 0.85 | 0.4644 | 1 |
| B8-Diet | 180 | 0.92 ± 0.18 | 7.09 (3.8–10.46) | 25.13 | <0.0001 | 0.2 | 0.8937 | 2.38 (1.02–5.58) |
| B9-Diet | 180 | 1.07 ± 0.31 | 8.84 (0.19–24.89) | 12.32 | 0.0004 | 2.63 | 0.0500 | 2.84 (1.16–6.95) |
| B10-Diet | 180 | 1.02 ± 0.39 | 4.9 (-) | 6.82 | 0.009 | 4.03 | 0.0070 | 1.65 (0.55–4.92) |
| B11-Diet | 180 | 0.84 ± 0.2 | 5.16 (2.03–8.25) | 18.68 | <0.0001 | 2.55 | 0.0539 | 1.73 (0.68–4.41) |
| B1-Vial | 180 | 6.89 ± 16,280.9 | 11.03 (-) | 0 | 0.9997 | 1.06 | 0.3653 | 257.89 (2.24E-104–2.96E-108) |
| B2-Vial | 180 | 0.58 ± 0.42 | 9.06 (-) | 1.89 | 0.1700 | 30.78 | <0.0001 | 194.52 (0.02–181,689.87) |
| B3-Vial | 180 | 6.17 ± 6,735.44 | 10.19 (-) | 0 | 0 | 0 | 0 | 248.34 (4.57E-12–1.35) |
| B4-Vial | 180 | 6.44 ± 13,161.97 | 10.34 (-) | 0 | 0 | 0 | 0 | 251.64 (1.48E-46–4.27E-50) |
| B5-Vial | 180 | 0.57 ± 0.1 | 2.95 (1.57–4.71) | 36.03 | <0.0001 | 0.79 | 0.4985 | 66.97 (0.0097–463,804.95) |
| B6-Vial | 180 | 0.55 ± 0.25 | 3.01 (-) | 4.84 | 0.0279 | 6.73 | 0.0002 | 73.72 (0.009–624,555.87) |
| B7-Vial | 180 | 0.84 ± 0.41 | 0.98 (-) | 4.21 | 0.0403 | 4.5 | 0.0037 | 24.01 (0.04–157.29) |
| B8-Vial | 180 | 0.97 ± 0.37 | 0.74 (0.04–1.33) | 6.84 | 0.0089 | 0.1 | 0.9574 | 7.84 (0.001–59,853.87) |
| B9-Vial | 180 | 1.17 ± 0.28 | 9.79 (5.57–13.77) | 18.07 | <0.0001 | 0.96 | 0.4112 | 206.08 (0.03–1,419,632.49) |
| B10-Vial | 180 | 0.32 ± 0.17 | 0.04 (-) | 3.72 | 0.0538 | 0.31 | 0.8152 | 1 |
| B11-Vial | 180 | 0.44 ± 0.3 | 0.60 (-) | 2.17 | 0.14 | 8.35 | <0.0001 | 9.59 (0.0007–14,1051.06) |
aLC50 and RR50 values are in μg per vial or diet cup; mortality was scored at 24 h.
bTest for slope significance indicates dose affects mortality.
cTest for goodness-of-fit (GoF) significance indicates error from probit trend is greater than expected for simple binomial response.
dRR50 (resistance ratio) and 95% CI calculated using formula from Robertson and Priestler (1992). Differences among SR50 values are significant if 95% CI do not include 1.0. RR50 compare the LC50s among the highest LC50 as a control.
(-)Values for CI not calculated.