Table 6.
Lethal mortality response (LC50) of Lygus lineolaris bioassayed using glass vial and diet cup with the insecticide thiamethoxam at different concentrations
| Bioassay (B)-No. | Concentration response (μg per vial or diet cup) | |||||||
|---|---|---|---|---|---|---|---|---|
| n | Slope ± SE | LC50 (95% CI)a | Probit trend | RR50 (95% CI)d | ||||
| Test for slopeb | Test for GoFc | |||||||
| Χ 2 | P > Χ 2 | Χ 2 | P > Χ 2 | |||||
| B1-Diet | 180 | 0.57 ± 0.16 | 2.38 (0.57–24.37) | 13.23 | 0.0003 | 4.04 | 0.007 | 1.14 (0.99–3.31) |
| B2-Diet | 180 | 0.48 ± 0.10 | 8.44 (3.97–15.56) | 20.94 | <0.0001 | 0.81 | 0.487 | 4.04 (0.74–4.56) |
| B3-Diet | 180 | 0.45 ± 0.11 | 8.27 (2.58–17.18) | 17.54 | <0.0001 | 1.86 | 0.1344 | 3.99 (0.71–5.82) |
| B4-Diet | 180 | 0.46 ± 0.13 | 3.95 (1.64–24.29) | 12.65 | 0.0004 | 0.44 | 0.7234 | 1.91 (0.93–19.74) |
| B5-Diet | 180 | 0.58 ± 0.18 | 2.15 (0.64–95.5) | 10.45 | 0.0012 | 5.35 | 0.0011 | 1.03 (0.91–4.78) |
| B6-Diet | 180 | 0.52 ± 0.12 | 8.54 (0.24–13.35) | 17.52 | <0.0001 | 1.19 | 0.3105 | 4.23 (2.22–3.77) |
| B7-Diet | 180 | 0.52 ± 0.08 | 2.07 (0.97–7.47) | 39.76 | <0.0001 | 2.02 | 0.1089 | 1 |
| B8-Diet | 180 | 0.47 ± 0.17 | 4.99 (-) | 7.57 | 0.0059 | 4.58 | 0.0033 | 2.44 (0.34–8.12) |
| B9-Diet | 180 | 0.46 ± 0.09 | 3.25 (0.44–34.71) | 28.32 | <0.0001 | 2.61 | 0.0494 | 1.57 (0.06–4.51) |
| B10-Diet | 180 | 0.82 ± 0.24 | 4.5 (2.21–10.3) | 12.01 | 0.0005 | 0.7 | 0.5500 | 1.96 (0.68–4.06) |
| B11-Diet | 180 | 1.25 ± 0.36 | 2.19 (0.36–7.26) | 12.24 | 0.0005 | 4.52 | 0.0036 | 1.07 (0.31–6.74) |
| B1-Vial | 180 | 0.24 ± 0.10 | 0.02(4.99E-14–0.22) | 5.43 | 0.0197 | 0.25 | 0.8631 | 1 |
| B2-Vial | 180 | 0.31 ± 0.12 | 0.04 (3.23E-7–0.28) | 6.91 | 0.0086 | 0.78 | 0.5069 | 2.32 (0.004–1,423.49) |
| B3-Vial | 180 | 1.22 ± 0.30 | 0.59 (0.35–0.81) | 16.65 | <0.0001 | 0.11 | 0.9600 | 67.55 (0.38–12,080.12) |
| B4-Vial | 180 | 0.64 ± 0.27 | 3.39 (-) | 5.53 | 0.0187 | 5.92 | 0.0005 | 334.43 (1.56–71,628.72) |
| B5-Vial | 180 | 0.46 ± 0.15 | 0.1 (0.0008–0.32) | 9.57 | 0.0020 | 1.09 | 0.3531 | 10.88 (0.04–2,719.04) |
| B6-Vial | 180 | 0.34 ± 0.13 | 0.03 (9.77E-8–0.22) | 6.63 | 0.0100 | 1.19 | 0.3100 | 3.65 (0.008–1,707.55) |
| B7-Vial | 180 | 4.55 ± 1.82 | 0.75 (0.6–2.8) | 6.25 | 0.0124 | 0.67 | 0.5694 | 92.23 (0.52–16,442.27) |
| B8-Vial | 180 | 0.3 ± 0.09 | 0.21 (0.005–0.7) | 11.97 | 0.0005 | 1.07 | 0.3584 | 18.93 (0.08–4,751.41) |
| B9-Vial | 180 | 0.54 ± 0.15 | 1.6 (0.0005–11.78) | 13.87 | 0.0002 | 2.98 | 0.0300 | 195.99 (0.94–40,711.31) |
| B10-Vial | 180 | 0.35 ± 0.34 | 56.23 (-) | 1.07 | 0.3001 | 2.62 | 0.0488 | 1,823.77 (4.56–733,498.33) |
| B11-Vial | 180 | 0.78 ± 0.31 | 0.19 (0.001–0.41) | 6.48 | 0.0100 | 0.17 | 0.9200 | 20.84 (0.10–4,362.07) |
aLC50 and RR50 values are in μg per vial or diet cup; mortality was scored at 48 h.
bTest for slope significance indicates dose affects mortality.
cTest for goodness-of-fit (GoF) significance indicates error from probit trend is greater than expected for simple binomial response.
dRR50 (resistance ratio) and 95% CI calculated using formula fromRobertson and Priestler (1992). Differences among SR50 values are significant if 95% CI do not include 1.0. RR50 compare the LC50s among the highest LC50 as a control.
(-)Values for CI not calculated.