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. 2020 Jul 13;20(4):4. doi: 10.1093/jisesa/ieaa067

Table 8.

Lethal mortality response (LC50) of Lygus lineolaris bioassayed using glass vial and diet cup with the insecticide sulfoxaflor at different concentrations

Bioassay (B)-No. Concentration response (μg per vial or diet cup)
n Slope ± SE LC50 (95% CI)a Probit trend RR50 (95% CI)d
Test for slopeb Test for GoFc
Χ 2 P > Χ 2 Χ 2 P > Χ 2
B1-Diet 180 1.85 ± 0.53 3.20 (09.01–4.05) 12.15 0.0005 0.71 0.5418 1.38 (0.91–7.26)
B2-Diet 180 1.59 ± 0.56 7.85 (2.08–4.75) 7.97 0.0048 2.35 0.0696 3.39 (1.33–6.79)
B3-Diet 180 0.56 ± 0.15 7.94 (2.08–15.36) 12.45 0.0004 0.50 0.6786 3.43 (0.31–6.79)
B4-Diet 180 0.88 ± 0.22 8.39 (1.91–28.01) 15.87 <0.0001 0.69 0.5538 3.61 (1.58–7.34)
B5-Diet 180 0.30 ± 0.74 9.09 (3.79–25.44) 16.49 <0.0001 0.47 0.6993 3.95 (0.58– 4.71)
B6-Diet 180 0.66 ± 0.19 2.37 (0.05–14.01) 11.37 0.0007 0.65 0.5806 1.06 (0.89–9.63)
B7-Diet 180 0.42 ± 0.08 2.31 (0.63–9.91) 26.68 <0.0001 0.30 0.8239 1
B8-Diet 180 0.76 ± 0.29 8.25 (-) 6.79 0.0092 2.43 0.0629 3.57 (0.63–5.56)
B9-Diet 180 0.61 ± 0.13 2.52 (6.76–20.23) 21.24 <0.0001 0.53 0.8588 1.07 (0.05–5.35)
B10-Diet 180 0.50 ± 0.17 4.32 (21.77–524.60) 8.17 0.0043 0.28 0.8361 1.89 (0.83–11.01)
B11-Diet 180 0.47 ± 0.10 8.52 (2.23–17.36) 15.23 <0.0001 0.99 0.3921 3.70 (0.62–5.24)
B1-Vial 180 0.53 ± 0.31 3.13 (-) 2.86 0.0911 4.53 0.0035 143.91 (0.44–46,766.95)
B2-Vial 180 2.05 ± 3.46 3.73 (-) 0.52 0.4699 0.25 0.8591 288.56 (1.50–55,532.61)
B3-Vial 180 0.20 ± 0.08 0.02 (2.13E-13–0.25) 5.55 0.0185 0.28 0.8398 1
B4-Vial 180 0.89 ± 0.22 6.17 (2.75–9.48) 16.37 <0.0001 0.92 0.4282 484.12 (2.98–78,518.96)
B5-Vial 180 1.10 ± 0.20 1.28 (0.84–1.79) 30.23 <0.0001 0.88 0.4458 109.64 (0.69–17,423.16)
B6-Vial 180 0.73 ± 0.13 6.95 (3.99–10.83) 30.35 <0.0001 1.17 0.3192 536.27 (3.33–86,183.4)
B7-Vial 180 0.74 ± 0.41 2.51 (-) 3.19 0.0739 7.86 <0.0001 191.47 (0.88–41,495.32)
B8-Vial 180 0.91 ± 0.68 1.85 (-) 1.78 0.1819 5.63 0.0007 95.53 (0.32–28,784.28)
B9-Vial 180 0.72 ± 0.11 1.47 (0.89–2.18) 38.8 <0.0001 0.22 0.8780 132.25 (0.83–21,130.05)
B10-Vial 180 0.51 ± 0.12 1.12 (0.23–2.57) 17.62 <0.0001 0.80 0.4929 24.68 (0.12–4,897.20)
B11-Vial 180 0.57 ± 0.16 3.50 (0.00–20.30) 12.23 0.0005 2.55 0.0536 211.87 (1.19–37,563.79)

aLC50 and RR50 values are in μg per vial or diet cup; mortality was scored at 72 h.

bTest for slope significance indicates dose affects mortality.

cTest for goodness-of-fit (GoF) significance indicates error from probit trend is greater than expected for simple binomial response.

dRR50 (resistance ratio) and 95% CI calculated using formula from Robertson and Priestler (1992). Differences amongst SR50 values are significant if 95% CI do not include 1.0. RR50 compare the LC50s among the highest LC50 as a control.

(-)Values for CI not calculated.