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. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Cell Rep. 2020 May 5;31(5):107599. doi: 10.1016/j.celrep.2020.107599

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
CaM Kinase II alpha (6G9) Novusbio # NB100–1983; RRID:AB_10001339
LMX1A Novusbio # NBP1–81303; RRID:AB_11008396
SYNAPSIN Synaptic System # 106 001; RRID:AB_887805
HOMER Synaptic System # 160 003; RRID:AB_887730
EGFR Abcam # Ab231; RRID:AB_2293306
FGFR1 phosphoY654 Abcam # Ab59194; RRID:AB_941585
TBR1 Abcam # Ab31940; RRID:AB_2200219
REELIN Abcam # Ab18570; RRID:AB_444539
CYCLIN D1 Abcam # Ab10540; RRID:AB_297280
FGFR2 Abcam # Ab10648; RRID:AB_297369
BMPR1A Abcam # Ab38560; RRID:AB_722713
HES1 Cell Signaling Technology # 11988; RRID:AB_2728766
p-SMAD1/5 Cell Signaling Technology # 9516; RRID:AB_491015
CYCLIN D1 Cell Signaling Technology # 2926; RRID:AB_2070400
pERK1/2 Cell Signaling Technology # 4370; RRID:AB_2315112
FGFR1 Cell Signaling Technology # 9740; RRID:AB_11178519
PAX6 BioLegend # PRB-278P; RRID:AB_291612
NESTIN R&D Systems # MAB1259; RRID:AB_2251304
OTX2 R&D Systems # AF1979; RRID:AB_2157172
PDGFR alpha R&D Systems # AF1062; # AF307; RRID:AB_2236897; RRID:AB_354459
SOX2 R&D Systems # AF2018; # MAB2018; RRID:AB_355110; RRID:AB_358009
SOX21 R&D Systems # AF3538; RRID:AB_2195947
TuJ1 R&D Systems # MAB1195; RRID:AB_357520
EGFR R&D Systems # AF1280; RRID:AB_354717
O4 R&D Systems # MAB 1326;RRID:AB_357617
GFAP DAKO # Z 0334; RRID:AB_10013382
HES5 Santa Cruz Biotechnology # sc-13859; RRID:AB_2233041
CUX1 Santa Cruz Biotechnology # sc-13024; RRID:AB_2261231
TLE4 Santa Cruz Biotechnology # sc-9125; RRID:AB_793141
FGFR3 Santa Cruz Biotechnology # sc-9007; RRID:AB_640487
LHX2 Santa Cruz Biotechnology # sc-19344; RRID:AB_2135660
FOXP2 Millipore # ABE73; RRID:AB_11214319
TBR1 Millipore # AB2261; RRID:AB_10615497
REELIN Millipore # MAB5366; RRID:AB_2285132
FOXG1 Takara/Clontech # M227; RRID:AB_2827749
GAD65/67 Kindly gifted by Dr. Christian Geis, Hans Berger Department of Neurology, Jena University Hospital, Germany (Hansen et al., 2013)
TBR2 Millipore # AB15894; RRID:AB_10615604
PAX6 Millipore # AB2237; RRID:AB_1587367
Alexa 488, 568, 647 conjugated antibodies Life Technologies N/A
Secondary antibodies donkey anti- (species) 488, 543, 647 Jackson DyLight N/A
Bacterial and Virus Strains
AAV5-CMV-eGFP Children’s Hospital of Philadelphia, Viral Vector Core Lot # RVC0041; titer:2.62E+13 vg/ml
AAV6-CMV-eGFP Children’s Hospital of Philadelphia, Viral Vector Core Lot # RVC0065; titer: 1.98E+12vg/ml
Biological Samples
Rhesus macaque monkey embryonic brain cells Primate breeding colony at Yale N/A
C57BL/6 mouse embryonic neural stem cells Charles River N/A
Sprague-Dawley rat embryonic astrocytes Charles River N/A
Chemicals, Peptides, and Recombinant Proteins
Poly-L-ornithine (PLO) Sigma # P3655
Fibronectin (FN) R&D Systems # 1030FN
DMEM/F12 medium Mediatech # 16–405-CV
Insulin Sigma # I6634
Apotransferrin Sigma # T2036
Progesterone Sigma # P8783
Putrescine Sigma # P5780
Sodium Selenite Sigma # S5261
bFGF R&D Systems # 4114-TC
NeuroBasal medium Life Technology # 12348–017
Glutamax Life Technology # 35050061
B27 Life Technology # 17504–044
BDNF R&D Systems # 248-BD
NT-3 R&D Systems # 267-N3
LDN193189 Stemgent # 04–0074
BMP4 R&D System # 5020-BP-010/CF
mTeSR1 Stem Cell Technology # 05850
ROCK inhibitor Sigma-Aldrich # Y0503
Matrigel BD # 354277
Aggrewell medium Stem Cell Technology # 05893
SB431542 Sigma-Aldrich # S4317
XAV939 Stemgent # 04–0046
FBS Life Technologies # 16000–044
Poly-D-lysine Sigma-Aldrich # P6407
DMEM Life Technologies # 11960069
Laminin Life Technologies # 23017–015
Papain BrainBits PAP
Critical Commercial Assays
microRNA booster kit Stemgent # 00–0073
StemFect RNA transfection reagent kit Stemgent # 00–0069
Alexa Fluor antibody labeling kit (488, 568, 647) Life Technologies # A20181; # A20184; # A20186
RNeasy Mini Kit QIAGEN # 74106
10X Genomics Single Cell 3“ RNA-Seq V2 10X Genomics N/A
Deposited Data
mouse RNaseq: in vitro E11.5 mouse cortical NSCs across neural differentiation
Series GSE144158 		This paper https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144158
human RNaseq #1: H9 hESC-derived NSCs serially passaged Series GSE144156 This paper https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144156
human RNaseq #2: 6 hiPSC lines [2063–1,−2; 2053–2,−6; 2075–1,−3] across neural differentiation Series GSE144157 This paper https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144157
single-cell RNA-sequencing of E77 and E78 Rhesus macaque Visual cortex (V1) Series GSE144508 This paper https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144508
Developing human DLPF cortex RNaseq dataset (Jaffe et al., 2018) http://eqtl.brainseq.org/phase1/
Laser micro-dissected macaque developing cortex microarray data (Bakken et al., 2016) http://www.blueprintnhpatlas.org/static/download
Human developing cortex single cell RNAseq data (Nowakowski et al., 2017) https://cells.ucsc.edu/?ds=cortex-dev
Fetal human cortex gene expression microarray data (Miller et al., 2014) http://www.brainspan.org/static/download.html
Cortecon (van de Leemput et al., 2014) http://cortecon.neuralsci.org/
Differentiating cortical neurons derived from hiPSCs (Ziller et al., 2015) https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE62193 (supplemental file “GSE62193_ AllSamples_ geneExpression.fpkm_tracking.gz”)
Experimental Models: Cell Lines
H9 human pluripotent stem cell line LIBD N/A
human induced pluripotent stem cell lines LIBD N/A
Experimental Models: Organisms/Strains
C57BL/6 mouse embryos Charles River N/A
Rhesus macaque monkey embryos Primate breeding colony at Yale N/A
Software and Algorithms
Columbus server Perkin Elmer N/A
Spotfire TIBCO N/A
pClamp10 software Molecular Devices N/A
Clampfit software Molecular Devices N/A
GraphPad Prism GraphPad Software N/A
BioCell SIMI Reality Motion Systems (Ravin et al., 2008)
Neurolucida software MBF Bioscience N/A
GWCoGAPS (Fertig et al., 2014; Stein-O’Brien et al., 2017) In the R statistical language: if (!requireNamespace(“BiocManager,” quietly = TRUE)) install. packages (“BiocManager”) BiocManager::
install(“CoGAPS”) browseVignettes (“CoGAPS”)
ProjectR (Sharma et al., 2020; Stein-O’Brien et al., 2019) In the R statistical language: if (!requireNamespace(“BiocManager,” quietly = TRUE)) install.packages (“BiocManager”) BiocManager:: install(“projectR”) browseVignettes (“projectR”)