Table 4.
Comparison of Current Assay with Published Sex Steroid Assaysa
| reference | method | derivative | analytes | LOD (fmol) | LOQ (fmol) | intra-assay CV (%) | linear dynamic range | tissues | species |
|---|---|---|---|---|---|---|---|---|---|
| current | LC/APCI/MS2 | - | DHEA, E2, T | 2, 3, 2 | 5, 10, 5 | <20 | to 1000 fmol | serum, liver, adipose | mouse |
| Denver et al., 20197 | LC/MS2 | MPPZ | E2 and 7 others | 0.77 | 1.6 | 7.6 | to 1595 fmol | plasma | human |
| Lee et al., 201623 | LC/MS2 | - | DHEA, E2, T,9 others | 35, 14, 73 | 5.1, 4.0, 7.5 | to 1000, 100, 50 ng/mL | serum | human | |
| Matysik et al., 201724 | LC/MS/HRMS | - | T, 7 others | 4 | 1−11.5 | serum | human | ||
| McCulloch et al., 20176 | LC/FF/APPI/MS2 | - | E2, T, 5 others | 3.7, 0.7 | serum | human | |||
| Schofield et al., 201725 | LC/MS2 | - | E2, T | 2.4, 4.6 | <20 | 6−600 pg/mL, 1−1170 ng/dL | serum | human | |
| Star-Weinstock et al., 201226 | LC/ESI/MS2 | quaternary aminooxy | T | 0.7 | <15 | 1−5000 pg/mL | serum | human | |
| Ankarberg-Lindgren et al., 201827 | GC/MS2 | PFBBr, PFBHA, PFPA | E2, T, 3 others | 0.05, 2.7 | <9 | serum | human | ||
| Gaikwad, 201328 | LC/MS2 | - | DHEA, E2, T, and 98 others | 87, 9, 9 | <19.7, <19.7, <8.8 | to 20 ng/μL | breast | human |
a
Blank spaces indicate that data are not available.