Figure 2. Stromal cells from olfactory epithelium tissue explants proliferated with DMEIW F12-CM. A-C) Flow cytometry histograms: Percentage of immunopositive CD29, CD73, and CD90 cells was indicative of mesenchymal stromal cells. Negative controls were labeled with the correspondent IgG isotype (black histograms). Dot blots (D-F) from stromal cells were negative for CD45 (not shown), and slightly positive for CD271 and CitK5. The percentage of immunopositive CD54/ICAM-1, p63, and nestin cells was indicative of horizontal (D and E) and ensheathing (F) antigens spontaneously expressed in mesenchymal stromal cells. All histograms are representative of one individual in a single analysis.