Figure 5. Human olfactory mucosa MSC mesenchymal neurospheres expressed the TUJ-1 protein of immature neurons. Differentiation of mesenchymal neurospheres from MSCs grown in coverslips in DMEM/F12-GF and DMEM/F12-RA + FORSK. MSCs were adapted to the lack of serum and seeded in DMEM/F12-G. Single cells from spheres were cultured in DMEM/F12-GF (A, C) and in DMEM/F12-RA+FORSK (B, D). Cells differentiated as mixed stroma and spheres (A, white arrow) or as predominant stroma (B). TUJ-1 (green) immunolabeling was found in spheres (C) and in single cells (D) from olfactory MSCs grown in coverslips in DMEM/F12-GF and DMEM/F12-RA + FORSK. Counterstaining was done with DAPI. Scale bar: A, B (100 um); C (50 µm); D (25 µm).