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. Author manuscript; available in PMC: 2021 May 20.
Published in final edited form as: Bioconjug Chem. 2020 Apr 23;31(5):1463–1473. doi: 10.1021/acs.bioconjchem.0c00156

Figure 1.

Figure 1.

(A) RP-HPLC analysis comparing MA-SML (+Ni2+) versus standard sortase-mediated ligation (−Ni2+) in a ligation reaction utilizing a 1:1 stoichiometry of a GGH-containing peptide substrate (1) and a diglycine nucleophile (GG-OH). Chromatograms represent the 6 h reaction timepoint. All peptide starting materials and products contained native carboxylic acid C-termini (−OH). (B) Time course of reactions in panel A demonstrating increased formation of ligation product 2 in the presence of Ni2+ as estimated by RP-HPLC. Data points represent three independent experiments (mean ± standard deviation).