FIG 3.
Expression of the PBAD and Pxut promoters in Pseudomonas species and E. coli over time with 10 mM inducer. Shown is the expression of gfpmut3 over time from the two different promoters in a pBBR1-based plasmid. Cultures were prepared in LB medium with an inducer (10 mM) and were grown at 30°C. Means and standard deviations are shown (n, ≥4 independent cultures for E. coli; n, 6 for Pseudomonas species). The pMQ578 plasmid has Pxut-gfp, and pMQ80 has PBAD-gfp. Xylose was used as an inducer for pMQ578, and arabinose was used for pMQ80. Asterisks indicate statistically significant differences (P < 0.05) between groups by Student’s t test.